Fluorescence-activated flow cytometry is one of the most powerful technologies that is routinely used in immunology. It allows not only for the rapid multiparametric analysis of cells on a ...

1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an interaction is ...

Celldetective is an open-source software integrating segmentation, tracking, and event detection to perform high-throughput end-to-end study of dynamic cell interactions, without requiring coding ...